Mobile and accurate detection system for infection by the 2009 pandemic influenza A (H1N1) virus with a pocket‐warmer reverse‐transcriptase loop‐mediated isothermal amplification
Identifieur interne : 000C79 ( Main/Exploration ); précédent : 000C78; suivant : 000C80Mobile and accurate detection system for infection by the 2009 pandemic influenza A (H1N1) virus with a pocket‐warmer reverse‐transcriptase loop‐mediated isothermal amplification
Auteurs : Ben Hatano [Japon, États-Unis] ; Megumi Goto [Japon] ; Hitomi Fukumoto [Japon] ; Takeyuki Obara [Japon] ; Takayuki Maki [Japon] ; Go Suzuki [Japon] ; Tetsuo Yamamoto [Japon] ; Kohsuke Hagisawa [Japon] ; Yoshitaro Matsushita [Japon] ; Tatsuya Fujii [Japon] ; Toshihiko Imakiire [Japon] ; Yuichi Kikuchi [Japon] ; Ryota Takahashi [Japon] ; Mie Kanai [Japon] ; Kaku Tamura [Japon] ; Tomoko Izumi [Japon] ; Yukihiro Takahashi [Japon] ; Yujiro Iwamoto [Japon] ; Satoshi Mimura [Japon] ; Yasuo Mukai [Japon] ; Kazue Takita [Japon] ; Hiroki Takeo [Japon] ; Ryuichi Kitamura [Japon] ; Eiichi Shimizu [Japon] ; Koji Fukushima [Japon] ; Yukiya Hakozaki [Japon] ; Akimi Uehata [Japon] ; Masao Sakai [Japon] ; Satoshi Ohshima [Japon] ; Toshiki Shirotani [Japon] ; Kunihiro Oba [Japon] ; Hideki Hasegawa [Japon] ; Tetsutaro Sata [Japon] ; Harutaka Katano [Japon]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2011-04.
English descriptors
- Teeft :
- Accurate diagnosis, Ambient light, Assay, Clin, Clin microbiol, Clin microbiol poon, Clinical samples, Copy number, Copy numbers, Disposable, Eiken, Eiken chemical, Extraction methods, Fatal cases, Hatano, Heat block, Heat blocks, Heating device, Immunochromatography, Immunochromatography assays, Immunochromatography tests, Infectious diseases, Inner primer, Isothermal, Isothermal assay, Japan force, Lamp method, Loop primer, Loop primers, Mobile system, National institute, Notomi, Pandemic, Positive samples, Positive signals, Present study, Primer, Quickvue tests, Rapid detection, Reaction tubes, Reagent, Respiratory syndrome, Seasonal viruses, Threshold turbidity level, Virol, Virus, Virus infection, Virus spread, Virus type.
Abstract
The 2009 pandemic H1N1 influenza A virus spread quickly worldwide in 2009. Since most of the fatal cases were reported in developing countries, rapid and accurate diagnosis methods that are usable in poorly equipped laboratories are necessary. In this study, a mobile detection system for the 2009 H1N1 influenza A virus was developed using a reverse‐transcriptase loop‐mediated isothermal amplification (RT‐LAMP) kit with a disposable pocket‐warmer as a heating device (designated as pwRT‐LAMP). The pwRT‐LAMP can detect as few as 100 copies of the virus—which is nearly as sensitive as real‐time reverse‐transcription polymerase chain reaction (RT‐PCR)—and does not cross‐react with RNA of seasonal influenza viruses. To evaluate the usefulness of the pwRT‐LAMP system, nasal swab samples were collected from 56 patients with flu‐like symptoms and were tested. Real‐time RT‐PCR confirmed that the 2009 H1N1 influenza A virus was present in 27 of the 56 samples. Of these 27 positive samples, QuickVue Influenza A + B immunochromatography detected the virus in only 11 samples (11/27; 40.7%), whereas the pwRT‐LAMP system detected the virus in 26 of the 56 samples (26/27 of the positive samples; 96.3%). These findings indicate that the mobile pwRT‐LAMP system is an accurate diagnostic system for the 2009 H1N1 influenza A virus, and has great potential utility in diagnosing future influenza pandemics. J. Med. Virol. 83:568–573, 2011. © 2011 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.22031
Affiliations:
Links toward previous steps (curation, corpus...)
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- to stream Istex, to step Curation: 000611
- to stream Istex, to step Checkpoint: 000086
- to stream Main, to step Merge: 000C81
- to stream Main, to step Curation: 000C79
Le document en format XML
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Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Maki, Takayuki" sort="Maki, Takayuki" uniqKey="Maki T" first="Takayuki" last="Maki">Takayuki Maki</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Military Medicine Research Unit, Test and Evaluation Command, Japan Ground Self‐Defense Force, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Suzuki, Go" sort="Suzuki, Go" uniqKey="Suzuki G" first="Go" last="Suzuki">Go Suzuki</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Military Medicine Research Unit, Test and Evaluation Command, Japan Ground Self‐Defense Force, Setagaya, Tokyo</wicri:regionArea><placeName><settlement 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type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Imakiire, Toshihiko" sort="Imakiire, Toshihiko" uniqKey="Imakiire T" first="Toshihiko" last="Imakiire">Toshihiko Imakiire</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kikuchi, Yuichi" sort="Kikuchi, Yuichi" uniqKey="Kikuchi Y" first="Yuichi" last="Kikuchi">Yuichi Kikuchi</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takahashi, Ryota" sort="Takahashi, Ryota" uniqKey="Takahashi R" first="Ryota" last="Takahashi">Ryota Takahashi</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kanai, Mie" sort="Kanai, Mie" uniqKey="Kanai M" first="Mie" last="Kanai">Mie Kanai</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tamura, Kaku" sort="Tamura, Kaku" uniqKey="Tamura K" first="Kaku" last="Tamura">Kaku Tamura</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Izumi, Tomoko" sort="Izumi, Tomoko" uniqKey="Izumi T" first="Tomoko" last="Izumi">Tomoko Izumi</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takahashi, Yukihiro" sort="Takahashi, Yukihiro" uniqKey="Takahashi Y" first="Yukihiro" last="Takahashi">Yukihiro Takahashi</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Iwamoto, Yujiro" sort="Iwamoto, Yujiro" uniqKey="Iwamoto Y" first="Yujiro" last="Iwamoto">Yujiro Iwamoto</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Mimura, Satoshi" sort="Mimura, Satoshi" uniqKey="Mimura S" first="Satoshi" last="Mimura">Satoshi Mimura</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Mukai, Yasuo" sort="Mukai, Yasuo" uniqKey="Mukai Y" first="Yasuo" last="Mukai">Yasuo Mukai</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takita, Kazue" sort="Takita, Kazue" uniqKey="Takita K" first="Kazue" last="Takita">Kazue Takita</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takeo, Hiroki" sort="Takeo, Hiroki" uniqKey="Takeo H" first="Hiroki" last="Takeo">Hiroki Takeo</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kitamura, Ryuichi" sort="Kitamura, Ryuichi" uniqKey="Kitamura R" first="Ryuichi" last="Kitamura">Ryuichi Kitamura</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Shimizu, Eiichi" sort="Shimizu, Eiichi" uniqKey="Shimizu E" first="Eiichi" last="Shimizu">Eiichi Shimizu</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Fukushima, Koji" sort="Fukushima, Koji" uniqKey="Fukushima K" first="Koji" last="Fukushima">Koji Fukushima</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Hakozaki, Yukiya" sort="Hakozaki, Yukiya" uniqKey="Hakozaki Y" first="Yukiya" last="Hakozaki">Yukiya Hakozaki</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Uehata, Akimi" sort="Uehata, Akimi" uniqKey="Uehata A" first="Akimi" last="Uehata">Akimi Uehata</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sakai, Masao" sort="Sakai, Masao" uniqKey="Sakai M" first="Masao" last="Sakai">Masao Sakai</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ohshima, Satoshi" sort="Ohshima, Satoshi" uniqKey="Ohshima S" first="Satoshi" last="Ohshima">Satoshi Ohshima</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Internal Medicine, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Shirotani, Toshiki" sort="Shirotani, Toshiki" uniqKey="Shirotani T" first="Toshiki" last="Shirotani">Toshiki Shirotani</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Clinical Technology Division, Japan Self‐Defense Force Central Hospital, Setagaya, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Oba, Kunihiro" sort="Oba, Kunihiro" uniqKey="Oba K" first="Kunihiro" last="Oba">Kunihiro Oba</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pediatrics, Showa General Hospital, Kodaira, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Hasegawa, Hideki" sort="Hasegawa, Hideki" uniqKey="Hasegawa H" first="Hideki" last="Hasegawa">Hideki Hasegawa</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Shinjuku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Musashimurayama, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sata, Tetsutaro" sort="Sata, Tetsutaro" uniqKey="Sata T" first="Tetsutaro" last="Sata">Tetsutaro Sata</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Shinjuku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Katano, Harutaka" sort="Katano, Harutaka" uniqKey="Katano H" first="Harutaka" last="Katano">Harutaka Katano</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Shinjuku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Japon</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Correspondence address: Department of Pathology, National Institute of Infectious Diseases, Tokyo 162‐8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">83</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="568">568</biblScope><biblScope unit="page" to="573">573</biblScope><biblScope unit="page-count">6</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2011-04">2011-04</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Accurate diagnosis</term><term>Ambient light</term><term>Assay</term><term>Clin</term><term>Clin microbiol</term><term>Clin microbiol poon</term><term>Clinical samples</term><term>Copy number</term><term>Copy numbers</term><term>Disposable</term><term>Eiken</term><term>Eiken chemical</term><term>Extraction methods</term><term>Fatal cases</term><term>Hatano</term><term>Heat block</term><term>Heat blocks</term><term>Heating device</term><term>Immunochromatography</term><term>Immunochromatography assays</term><term>Immunochromatography tests</term><term>Infectious diseases</term><term>Inner primer</term><term>Isothermal</term><term>Isothermal assay</term><term>Japan force</term><term>Lamp method</term><term>Loop primer</term><term>Loop primers</term><term>Mobile system</term><term>National institute</term><term>Notomi</term><term>Pandemic</term><term>Positive samples</term><term>Positive signals</term><term>Present study</term><term>Primer</term><term>Quickvue tests</term><term>Rapid detection</term><term>Reaction tubes</term><term>Reagent</term><term>Respiratory syndrome</term><term>Seasonal viruses</term><term>Threshold turbidity level</term><term>Virol</term><term>Virus</term><term>Virus infection</term><term>Virus spread</term><term>Virus type</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The 2009 pandemic H1N1 influenza A virus spread quickly worldwide in 2009. Since most of the fatal cases were reported in developing countries, rapid and accurate diagnosis methods that are usable in poorly equipped laboratories are necessary. In this study, a mobile detection system for the 2009 H1N1 influenza A virus was developed using a reverse‐transcriptase loop‐mediated isothermal amplification (RT‐LAMP) kit with a disposable pocket‐warmer as a heating device (designated as pwRT‐LAMP). The pwRT‐LAMP can detect as few as 100 copies of the virus—which is nearly as sensitive as real‐time reverse‐transcription polymerase chain reaction (RT‐PCR)—and does not cross‐react with RNA of seasonal influenza viruses. To evaluate the usefulness of the pwRT‐LAMP system, nasal swab samples were collected from 56 patients with flu‐like symptoms and were tested. Real‐time RT‐PCR confirmed that the 2009 H1N1 influenza A virus was present in 27 of the 56 samples. Of these 27 positive samples, QuickVue Influenza A + B immunochromatography detected the virus in only 11 samples (11/27; 40.7%), whereas the pwRT‐LAMP system detected the virus in 26 of the 56 samples (26/27 of the positive samples; 96.3%). These findings indicate that the mobile pwRT‐LAMP system is an accurate diagnostic system for the 2009 H1N1 influenza A virus, and has great potential utility in diagnosing future influenza pandemics. J. Med. Virol. 83:568–573, 2011. © 2011 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Japon</li><li>États-Unis</li></country><region><li>Région de Kantō</li><li>Virginie</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Hatano, Ben" sort="Hatano, Ben" uniqKey="Hatano B" first="Ben" last="Hatano">Ben Hatano</name></region><name sortKey="Fujii, Tatsuya" sort="Fujii, Tatsuya" uniqKey="Fujii T" first="Tatsuya" last="Fujii">Tatsuya Fujii</name><name sortKey="Fukumoto, Hitomi" sort="Fukumoto, Hitomi" uniqKey="Fukumoto H" first="Hitomi" last="Fukumoto">Hitomi Fukumoto</name><name sortKey="Fukumoto, Hitomi" sort="Fukumoto, Hitomi" uniqKey="Fukumoto H" first="Hitomi" last="Fukumoto">Hitomi Fukumoto</name><name sortKey="Fukushima, Koji" sort="Fukushima, Koji" uniqKey="Fukushima K" first="Koji" last="Fukushima">Koji Fukushima</name><name sortKey="Goto, Megumi" sort="Goto, Megumi" uniqKey="Goto M" first="Megumi" last="Goto">Megumi Goto</name><name sortKey="Hagisawa, Kohsuke" sort="Hagisawa, Kohsuke" uniqKey="Hagisawa K" first="Kohsuke" last="Hagisawa">Kohsuke Hagisawa</name><name sortKey="Hakozaki, Yukiya" sort="Hakozaki, Yukiya" uniqKey="Hakozaki Y" first="Yukiya" last="Hakozaki">Yukiya Hakozaki</name><name sortKey="Hasegawa, Hideki" sort="Hasegawa, Hideki" uniqKey="Hasegawa H" first="Hideki" last="Hasegawa">Hideki Hasegawa</name><name sortKey="Hasegawa, Hideki" sort="Hasegawa, Hideki" uniqKey="Hasegawa H" first="Hideki" last="Hasegawa">Hideki Hasegawa</name><name sortKey="Hatano, Ben" sort="Hatano, Ben" uniqKey="Hatano B" first="Ben" last="Hatano">Ben Hatano</name><name sortKey="Imakiire, Toshihiko" sort="Imakiire, Toshihiko" uniqKey="Imakiire T" first="Toshihiko" last="Imakiire">Toshihiko Imakiire</name><name sortKey="Iwamoto, Yujiro" sort="Iwamoto, Yujiro" uniqKey="Iwamoto Y" first="Yujiro" last="Iwamoto">Yujiro Iwamoto</name><name sortKey="Izumi, Tomoko" sort="Izumi, Tomoko" uniqKey="Izumi T" first="Tomoko" last="Izumi">Tomoko Izumi</name><name sortKey="Kanai, Mie" sort="Kanai, Mie" uniqKey="Kanai M" first="Mie" last="Kanai">Mie Kanai</name><name sortKey="Katano, Harutaka" sort="Katano, Harutaka" uniqKey="Katano H" first="Harutaka" last="Katano">Harutaka Katano</name><name sortKey="Katano, Harutaka" sort="Katano, Harutaka" uniqKey="Katano H" first="Harutaka" last="Katano">Harutaka Katano</name><name sortKey="Katano, Harutaka" sort="Katano, Harutaka" uniqKey="Katano H" first="Harutaka" last="Katano">Harutaka Katano</name><name sortKey="Kikuchi, Yuichi" sort="Kikuchi, Yuichi" uniqKey="Kikuchi Y" first="Yuichi" last="Kikuchi">Yuichi Kikuchi</name><name sortKey="Kitamura, Ryuichi" sort="Kitamura, Ryuichi" uniqKey="Kitamura R" first="Ryuichi" last="Kitamura">Ryuichi Kitamura</name><name sortKey="Maki, Takayuki" sort="Maki, Takayuki" uniqKey="Maki T" first="Takayuki" last="Maki">Takayuki Maki</name><name sortKey="Matsushita, Yoshitaro" sort="Matsushita, Yoshitaro" uniqKey="Matsushita Y" first="Yoshitaro" last="Matsushita">Yoshitaro Matsushita</name><name sortKey="Mimura, Satoshi" sort="Mimura, Satoshi" uniqKey="Mimura S" first="Satoshi" last="Mimura">Satoshi Mimura</name><name sortKey="Mukai, Yasuo" sort="Mukai, Yasuo" uniqKey="Mukai Y" first="Yasuo" last="Mukai">Yasuo Mukai</name><name sortKey="Oba, Kunihiro" sort="Oba, Kunihiro" uniqKey="Oba K" first="Kunihiro" last="Oba">Kunihiro Oba</name><name sortKey="Obara, Takeyuki" sort="Obara, Takeyuki" uniqKey="Obara T" first="Takeyuki" last="Obara">Takeyuki Obara</name><name sortKey="Ohshima, Satoshi" sort="Ohshima, Satoshi" uniqKey="Ohshima S" first="Satoshi" last="Ohshima">Satoshi Ohshima</name><name sortKey="Sakai, Masao" sort="Sakai, Masao" uniqKey="Sakai M" first="Masao" last="Sakai">Masao Sakai</name><name sortKey="Sata, Tetsutaro" sort="Sata, Tetsutaro" uniqKey="Sata T" first="Tetsutaro" last="Sata">Tetsutaro Sata</name><name sortKey="Shimizu, Eiichi" sort="Shimizu, Eiichi" uniqKey="Shimizu E" first="Eiichi" last="Shimizu">Eiichi Shimizu</name><name sortKey="Shirotani, Toshiki" sort="Shirotani, Toshiki" uniqKey="Shirotani T" first="Toshiki" last="Shirotani">Toshiki Shirotani</name><name sortKey="Suzuki, Go" sort="Suzuki, Go" uniqKey="Suzuki G" first="Go" last="Suzuki">Go Suzuki</name><name sortKey="Takahashi, Ryota" sort="Takahashi, Ryota" uniqKey="Takahashi R" first="Ryota" last="Takahashi">Ryota Takahashi</name><name sortKey="Takahashi, Yukihiro" sort="Takahashi, Yukihiro" uniqKey="Takahashi Y" first="Yukihiro" last="Takahashi">Yukihiro Takahashi</name><name sortKey="Takeo, Hiroki" sort="Takeo, Hiroki" uniqKey="Takeo H" first="Hiroki" last="Takeo">Hiroki Takeo</name><name sortKey="Takita, Kazue" sort="Takita, Kazue" uniqKey="Takita K" first="Kazue" last="Takita">Kazue Takita</name><name sortKey="Tamura, Kaku" sort="Tamura, Kaku" uniqKey="Tamura K" first="Kaku" last="Tamura">Kaku Tamura</name><name sortKey="Uehata, Akimi" sort="Uehata, Akimi" uniqKey="Uehata A" first="Akimi" last="Uehata">Akimi Uehata</name><name sortKey="Yamamoto, Tetsuo" sort="Yamamoto, Tetsuo" uniqKey="Yamamoto T" first="Tetsuo" last="Yamamoto">Tetsuo Yamamoto</name></country><country name="États-Unis"><region name="Virginie"><name sortKey="Hatano, Ben" sort="Hatano, Ben" uniqKey="Hatano B" first="Ben" last="Hatano">Ben Hatano</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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